Bicol University R&D Journal

Official Refereed Journal of Bicol University

4500 Legazpi City,Philippines

Print ISSN: 0016-4139       Online ISSN: 2719-082X

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Vol 26, Issue 1
August 2023

CHED-JIP Recognized Journal as per CMO NO. 50, s. 2018.

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Biology

GC-MS Analysis of Metabolites from Aspergillus tamarii and Trichoderma sp. Detected Promising Biological Compounds

Publisher: Bicol University
Abstract

Fifteen multicellular fungi previously isolated from rice paddies were evaluated for their antibacterial activities against test organisms using the agar plug method. Only seven of the fungal isolates showed activity against the test organisms Escherichia coli, Bacillus cereus, and Staphylococcus aureus. These seven fungi were subsequently grown on yeast extract sucrose agar (YES), potato dextrose agar (PDA), and Sabouraud dextrose agar (SDA) to determine their radial growth and time of sporulation over a period of seven days. The fastest sporulation was achieved by Penicillium funiculosum (three days), Aspergillus tamarii (five days), and Trichoderma sp. (six days). Spore suspensions (150 spores/mL) of these fungi were separately inoculated in 100 mL of Yeast Extract Broth (YEB), Potato Dextrose Broth (PDB), and Sabouraud Dextrose Broth (SDB), and cultured for 35 days in room temperature (24–27o C) at stationary condition. Culture filtrates were extracted with ethyl acetate (1:1 v/v) and used for herbicidal assay using Vigna radiata and for gas chromatography-mass spectrometry (GC-MS). No herbicidal activity was observed with water as control. Compounds identified (>90% similarity with National Institute for Standards and Technology [NIST] library) included bis (2-ethyl hexyl) phthalate (antifungal and anti-inflammatory), dehydromevalonic lactone (fragrance production), benzene acetaldehyde (fragrance production), and n-hexadecanoic acid (anti-inflammatory). Metabolomics data provide the basis for future optimization studies to generate utilizable bioactive compounds


Charmaine A. Malonzo
Jonathan Jaime G. Guerrero
Maria Czet Fulleros
Ramil Earl A. Madrideo

GC-MS
secondary metabolites
multicellular fungi
Aspergillus tamarii
Trichoderma sp

December 10, 2018
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CHED-JIP Recognized Journal as per CMO NO. 50, s. 2018.

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Higher Education Regional Research Center, Bicol University East Campus, Legazpi City, Albay, Philippines

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