Bicol University R&D Journal

Official Refereed Journal of Bicol University

4500 Legazpi City,Philippines

Print ISSN: 0016-4139       Online ISSN: 2719-082X

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Vol 26, Issue 1
August 2023

CHED-JIP Recognized Journal as per CMO NO. 50, s. 2018.

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Biology

In Vitro Nematicidal Activities of Myxobacteria from Mt. Malinao, Philippines Against Caenorhabditis elegans

Publisher: Bicol University
Abstract

Secondary metabolites from microorganisms are good sources of natural products which can be utilized for various medical and biotechnological purposes. Several members of the bacterial group Myxobacteria are known to be prolific producers of secondary metabolites. Philippine forest soils are rich habitats for Myxobacteria which could produce secondary metabolites with potential novel biological activities. Soils from Mt. Makiling in Laguna and from Mt. Malinao in Albay were collected for the isolation of myxobacteria which were then screened for nematicidal activities. The dung bait plate, yeast-spot plate and coli-spot plate baiting techniques were used to isolate myxobacteria from soils. Myxobacterial isolates were selected based on the presence of fruiting bodies and swarm colonies characteristic of myxobacteria. Ten representative isolates were grown in CTT medium (30ºC, 150 rpm, four days) with 2% XAD-16 adsorber resin. Cell metabolites were extracted using 1:1 methanol:acetone. The crude extracts were used for the nematicidal assay against Caenorhabditis elegans Bristol N2 for 24 hours at 25ºC. High nematicidal activities based on nematode mortality were observed from the extracts of L4D1 (90.33% +/- 3.06) and L4D9 (86.00% +/- 3.6) while the commercial preparation of mebendazole as positive control showed 100% mortality. The differences in the percentage mortalities caused by the various extracts were highly significant at p<0.05 based on One Way ANOVA and Tukey HSD. L4D9 and L4D1 were identified based on phenotypes and 16S rRNA sequences as strains of Myxococcus xanthus. This study contributes to the scarce literature and culture collection of myxobacteria in the Philippines and especially those with potential anthelminthic activities.


Charmaine A. Malonzo
Bernadette C. Mendoza
Rosario G. Monsalud
Ida F. Dalmacio
Nacita B. Lantican

secondary metabolites
Mt. Makiling
Mt. Malinao
nematicide
Myxococcus

July 25, 2022
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CHED-JIP Recognized Journal as per CMO NO. 50, s. 2018.

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Higher Education Regional Research Center, Bicol University East Campus, Legazpi City, Albay, Philippines

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